FENS Forum 2004

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First author: Ponti, Giovanna (poster)

Poster 18 - Tue 13/07, 11:30 - Hall I
Session 145 - Developmental cell proliferation
Abstract A145.18, published in FENS Forum Abstracts, vol. 2, 2004.
Ref.: FENS Abstr., vol.2, A145.18, 2004

Author(s) Ponti G. (1), Peretto P. (2) & Bonfanti L. (1)

Addresse(s) (1) Dip. Morfofisiologia Veterinaria, Turin, Italy; (2) Dip. Biologia Animale, Turin, Italy

Title Genesis of neuronal precursors in the cerebellar cortex of the adult rabbit.

Text In the mammalian central nervous system adult neurogenesis is restricted to certain brain areas. In the cerebellum a delayed genesis of granule cells takes place from the external granule layer (EGL), which is known to disappear early postnatally. By using immunocytochemistry for cell proliferation antigens (Ki67, BrdUrd) and markers typically associated to newly-generated neuronal precursors (PSA-NCAM, beta-tubulin, doublecortin) we report the occurrence of consistent cell genesis in the cerebellum of adult rabbits. Cell proliferation was concentrated in a monolayer localized beneath the pia mater of the whole cerebellum (subpial layer, SPL). The SPL was present in all animals examined (3 to 6 months old) denying the disappearance of the EGL after the first postnatal month. Electron microscopy confirmed the presence of mitosis and cells with the cytology of neuroblasts in the SPL, at the interface between the basal lamina and the molecular layer. Quantification of BrdUrd+ cells at 2 hours, 5 and 15 days survival after administration showed a progressive decrease in the cell number within the SPL and an increase in cortical layers, suggesting their radial migration. PSA-NCAM and doublecortin immunostainings identified bipolar shaped cells and bipolar cells with some branches in both layers, and neuronal-like cells in the molecular layer. These latter does not correspond to any known cerebellar cell type. BrdUrd/PSA-NCAM double stainings performed at different survival times confirmed that these cells are newly generated in a centripethal neurogenetic sequence. These results show that, unlike rodents, the rabbit cerebellum retains the capacity to undergo cell genesis during adulthood. The data at present available suggest that a progeny of neuronal precursors reach the cerebellar cortical layers giving rise to neurons in the molecular layer, thus not following the same pattern of postnatal granule cell precursors.

Theme Development
Neurogenesis - Gliogenesis / Proliferation

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Author(s)	Ponti G. (1), Peretto P. (2) & Bonfanti L. (1)
Addresse(s)	(1) Dip. Morfofisiologia Veterinaria, Turin, Italy; (2) Dip. Biologia Animale, Turin, Italy
Title	Genesis of neuronal precursors in the cerebellar cortex of the adult rabbit.
Text	In the mammalian central nervous system adult neurogenesis is restricted to certain brain areas. In the cerebellum a delayed genesis of granule cells takes place from the external granule layer (EGL), which is known to disappear early postnatally. By using immunocytochemistry for cell proliferation antigens (Ki67, BrdUrd) and markers typically associated to newly-generated neuronal precursors (PSA-NCAM, beta-tubulin, doublecortin) we report the occurrence of consistent cell genesis in the cerebellum of adult rabbits. Cell proliferation was concentrated in a monolayer localized beneath the pia mater of the whole cerebellum (subpial layer, SPL). The SPL was present in all animals examined (3 to 6 months old) denying the disappearance of the EGL after the first postnatal month. Electron microscopy confirmed the presence of mitosis and cells with the cytology of neuroblasts in the SPL, at the interface between the basal lamina and the molecular layer. Quantification of BrdUrd+ cells at 2 hours, 5 and 15 days survival after administration showed a progressive decrease in the cell number within the SPL and an increase in cortical layers, suggesting their radial migration. PSA-NCAM and doublecortin immunostainings identified bipolar shaped cells and bipolar cells with some branches in both layers, and neuronal-like cells in the molecular layer. These latter does not correspond to any known cerebellar cell type. BrdUrd/PSA-NCAM double stainings performed at different survival times confirmed that these cells are newly generated in a centripethal neurogenetic sequence. These results show that, unlike rodents, the rabbit cerebellum retains the capacity to undergo cell genesis during adulthood. The data at present available suggest that a progeny of neuronal precursors reach the cerebellar cortical layers giving rise to neurons in the molecular layer, thus not following the same pattern of postnatal granule cell precursors.
Theme	Development Neurogenesis - Gliogenesis / Proliferation